Polarography of 5,5'-dithio bis-(2 nitrobenzoic acid) in the pH range 2-12, in Britton-Robinson buffer at constant ionic strength (0 .5 M) has been carried out. Four cathodic waves (A, B, C and D) and an anodic wave have been obtained . Wave A is produced by a reduction of pre-protonated nitro group
Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination
✍ Scribed by Peter W. Riddles; Robert L. Blakeley; Burt Zerner
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 586 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Hydrogen sulfide was determined with N-ethylmaleimide (NEM), p-chloromercuribenzoate (PCMB), and 5,5'-dithiobiQ2nitrobenzoic acid) (DTNB). One mole of hydrogen sulfide reacted with two moles of NEM or PCMB. It reacted with only one mole of DTNB, but produced two moles of the thiol anion, 5thio-2nitr
The primary reaction between the cysteine residues of histone H3 and dithiobis-(nitrobenzoic acid) may be succeeded by thiol-disulfide interchange steps leading to intraor intermolecularly crosslinked H3 molecules. A chromatographic assay is applied to detect consecutive reactions of this type and i
A new reagent, 6,6'-diselenobis-(3nitrobenzoic acid) (DSNB) has been synthesized and is shown to be useful for quantitative estimation of sulfhydryl groups in proteins. This reagent, which is a selenium analog of Ellman's reagent, reacts specifically and quantitatively with thiol groups of proteins