Elicitation of thiophene production by cultured hairy roots ofTagetes patula
β Scribed by T. Rajasekaran; R. Madhusudan; G. A. Ravishankar
- Book ID
- 107480985
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 378 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0137-5881
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Marigold (Tagetes patula L.) hairy roots induced by infection with Agrobacterium rhizogenes produced Ξ±-terthienyl when grown in darkness, and an n-hexane extract of the roots showed nematocidal activity. Depending on the hairy root line used, the level of Ξ±-terthienyl varied from 15 to 1268 ΞΌg per g
The effect of different concentrations of carbohydrates, nitrogen, sulphate, plant growth regulators and elicitors on growth and thiophene accumulation by transformed root cultures of Tagetes iaxa (Cabrera) was studied. The combinations of sucrose (30 g/l), nitrogen (60 raM), sulphate (150 raM) and
Hairy roots of Tagetes patula were grown for 24 days in modified Murashige and Skoog's liquid medium at different initial pH levels of 4.0, 5.0, 5.7, 6.0 and 7.0. Irrespective of the initial pH, after 12 days, the pH of the culture medium was approximately 4.5. However the final pH, after 24 days of