In vitro thiophene production by transformed root cultures ofTagetes laxa (Cabrera)

✍ Scribed by J. Rodríguez Talou; A. M. Giulietti

• Book ID: 104656608
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 362 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00189222

✦ Synopsis

The effect of different concentrations of carbohydrates, nitrogen, sulphate, plant growth regulators and elicitors on growth and thiophene accumulation by transformed root cultures of Tagetes iaxa (Cabrera) was studied. The combinations of sucrose (30 g/l), nitrogen (60 raM), sulphate (150 raM) and the ratio Nox:Nred 2:1 are the most appropriate combination to support growth and thiophene accumulation, which was increased by 90% when the cultures wore elicited with homogenate of Sclerotinia sclerottiorum . The plant growth regulators used produced dedifferentiation with a decrease in thiophene biosynthesis.