Hairy roots of Tagetes patula were grown for 24 days in modified Murashige and Skoog's liquid medium at different initial pH levels of 4.0, 5.0, 5.7, 6.0 and 7.0. Irrespective of the initial pH, after 12 days, the pH of the culture medium was approximately 4.5. However the final pH, after 24 days of
Production of nematocidal compounds by hairy root cultures ofTagetes patulaL.
✍ Scribed by Masaharu Kyo; Yukio Miyauchi; Tadaaki Fujimoto; Shigeyuki Mayama
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 347 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
Marigold (Tagetes patula L.) hairy roots induced by infection with Agrobacterium rhizogenes produced α-terthienyl when grown in darkness, and an n-hexane extract of the roots showed nematocidal activity. Depending on the hairy root line used, the level of α-terthienyl varied from 15 to 1268 μg per g dry weight, a level that corresponded to 0.15 to 12.7-fold that in intact roots. Analysis by HPLC indicated that the nematocidal activity was due predominantly to α-terthienyl. However, it is suggested that nematocidal compounds other than α-terthienyl are present in hairy roots cultured in the dark for long periods or in the light.
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