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Production of nematocidal compounds by hairy root cultures ofTagetes patulaL.

✍ Scribed by Masaharu Kyo; Yukio Miyauchi; Tadaaki Fujimoto; Shigeyuki Mayama


Publisher
Springer
Year
1990
Tongue
English
Weight
347 KB
Volume
9
Category
Article
ISSN
0721-7714

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✦ Synopsis


Marigold (Tagetes patula L.) hairy roots induced by infection with Agrobacterium rhizogenes produced α-terthienyl when grown in darkness, and an n-hexane extract of the roots showed nematocidal activity. Depending on the hairy root line used, the level of α-terthienyl varied from 15 to 1268 μg per g dry weight, a level that corresponded to 0.15 to 12.7-fold that in intact roots. Analysis by HPLC indicated that the nematocidal activity was due predominantly to α-terthienyl. However, it is suggested that nematocidal compounds other than α-terthienyl are present in hairy roots cultured in the dark for long periods or in the light.


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