Electrospray Ionization Mass Spectrometry: Part II: Applications in Characterization of Peptides and Proteins
β Scribed by Bakhtiar, Ray; Hofstadler, Steven A.; Smith, Richard D.
- Book ID
- 111680652
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 546 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0021-9584
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Bacterioopsin (BO) from Halobacterium halobium, as well as its V8-protease and CNBr fragments from the C-terminal region, were used to establish appropriate conditions for the mass determination of membrane proteins and peptides by electrospray mass spectrometry (ESI-MS). Of the tested solvents neat
Capillary zone electrophoresis (CZE) coupled with an on-line mass spectrometric (MS) detection system by an atmospheric pressure electrospray ionization (ESI) interface has been used successfully for separations of peptide and protein mixtures. For CZE-MS, ESI produces multiply charged molecular ion