Capillary zone electrophoresis–mass spectrometry with electrospray ionization of peptides and proteins

✍ Scribed by Joseph A. Loo; Harlan K. Jones; Harold R. Udseth; Richard D. Smith

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 504 KB
• Volume: 1
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220010504

✦ Synopsis

Capillary zone electrophoresis (CZE) coupled with an on-line mass spectrometric (MS) detection system by an atmospheric pressure electrospray ionization (ESI) interface has been used successfully for separations of peptide and protein mixtures. For CZE-MS, ESI produces multiply charged molecular ions and thus allows m a s spectrometers with a limited mass-to-charge range to analyze proteins having molecular weights greater than 100,000. Various pH buffer systems have been investigated, including buffer systems in which pH > p1 (isoelectric point of analytes) and pH << pl are designed to reduce protein adsorption onto silica capillary surfaces and thus improve separation efficiencies. More than 125,000 theoretical plates have been obtained from a CZE-MS separation of less than 1 pmol per component of leucine enkephalin and horse heart myoglobin. CZE-MS of other large peptides and proteins are demonstrated.