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Electrophoretic and immunoblot analysis of Campylobacter fetus lipopolysaccharides

โœ Scribed by B.W. Brooks; M.M. Garcia; R.H. Robertson; H. Lior

Book ID
113362022
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
833 KB
Volume
51
Category
Article
ISSN
0378-1135

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A procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (LPS) from SDS-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. The method is sensitive, and reveals and resolves high molecular weight LPS molec