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Analysis of electrophoretically heterogeneous lipopolysaccharides of Escherichia coli by immunoblotting

โœ Scribed by Helge Karch; Hermann Leying; Wolfgang Opferkuch


Book ID
109312511
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
396 KB
Volume
22
Category
Article
ISSN
0378-1097

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A procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (LPS) from SDS-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. The method is sensitive, and reveals and resolves high molecular weight LPS molec