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Immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide

โœ Scribed by S. Sturm; P. Fortnagel; K. N. Timmis

Publisher
Springer
Year
1984
Tongue
English
Weight
707 KB
Volume
140
Category
Article
ISSN
0302-8933

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โœฆ Synopsis

A procedure is described for the efficient transfer of fractionated bacterial lipopolysaccharide (LPS) from SDS-polyacrylamide gels to nitrocellulose filters, and its subsequent display by a peroxidase-linked antibody. The method is sensitive, and reveals and resolves high molecular weight LPS molecules having side chain lengths of up to and greater than 30 repeat units. It is useful for the rapid analysis of LPS in bacterial outer membrane preparations.

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โœ D. Cammaerts; M. Jacobs ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 907 KB

A rapid and convenient method is described for resolving the polypeptide composition of Fraction 1 protein. Using crude leaf extracts of a number of Lycopersicon species, Fraction 1 protein was first separated by polyacrylamide gel electrophoresis and the gel slices containing the protein were isoel