A simple electrophoretic procedure for the determination of the polypeptide composition of the subunits of Fraction 1 protein

✍ Scribed by D. Cammaerts; M. Jacobs

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 907 KB
• Volume: 109
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90654-5

✦ Synopsis

A rapid and convenient method is described for resolving the polypeptide composition of Fraction 1 protein. Using crude leaf extracts of a number of Lycopersicon species, Fraction 1 protein was first separated by polyacrylamide gel electrophoresis and the gel slices containing the protein were isoelectrofocused in the presence of 8 M urea. Isoelectric focusing was also applied directly on subunits in gel slices obtained after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The polypeptide composition produced is in agreement with previous determinations obtained by more elaborated techniques. ' This study was supported by a&ant ofthe NFWO. Belgium.

MATERIALS

Leaf homogenates of a few Lycopersicon species were used to perform the experiments. Seeds were kindly provided by Professor C.