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Electrolytic preparation of a reduced ninhydrin reagent for amino acid analysis

✍ Scribed by Toshitaka Hori; Sorin Kihara

Book ID: 112327912
Publisher: Springer
Year: 1988
Tongue: English
Weight: 359 KB
Volume: 330
Category: Article
ISSN: 1618-2650
DOI: 10.1007/bf00473781

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The reaction with indane-1,2,3-trione-2-hydrate (ninhydrin) is firmly established for the calorimetric determination of a-amino acids (l-3). The intensity of the blue-violet color produced is measured at about 570 rnp. This color is most intense when the reaction with ninhydrin is carried out at abo