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Electrogenic pump current of sarcoplasmic reticulum Ca2+-ATPase reconstituted at high lipid/protein ratio

✍ Scribed by Flemming Cornelius; Jesper V. Møller


Book ID
115925029
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
499 KB
Volume
284
Category
Article
ISSN
0014-5793

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A detailed methodology is presented of two reconstitution protocols for the (Ca(2+)-Mg2+)-ATPase from rabbit skeletal muscle, using the detergent potassium cholate. Method A was shown to produce fully fragmented membranes of definable lipid to protein ratios, which were unable to take up calcium upo