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Dependence of the fluorescence of fluorescein labelled (Ca2+, Mg2+)-ATPase upon the lipid to protein ratio in sarcoplasmic reticulum reconstituted systems

✍ Scribed by Ana M. Mata; Carlos Gutiérrez-Merino

Book ID
115759144
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
444 KB
Volume
133
Category
Article
ISSN
0006-291X

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A detailed methodology is presented of two reconstitution protocols for the (Ca(2+)-Mg2+)-ATPase from rabbit skeletal muscle, using the detergent potassium cholate. Method A was shown to produce fully fragmented membranes of definable lipid to protein ratios, which were unable to take up calcium upo