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Electrical pump currents generated by the Ca2+-ATPase of sarcoplasmic reticulum vesicles adsorbed on black lipid membranes

โœ Scribed by K. Hartung; E. Grell; W. Hasselbach; E. Bamberg


Book ID
115728187
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
957 KB
Volume
900
Category
Article
ISSN
0005-2736

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A detailed methodology is presented of two reconstitution protocols for the (Ca(2+)-Mg2+)-ATPase from rabbit skeletal muscle, using the detergent potassium cholate. Method A was shown to produce fully fragmented membranes of definable lipid to protein ratios, which were unable to take up calcium upo