Electrochemical Determination of L-Lactate Using Phenylboronic Acid Monolayer-Modified Electrodes

✍ Scribed by Shigehiro Takahashi; Shiho Kurosawa; Jun-ichi Anzai

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 150 KB
• Volume: 20
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200704097

✦ Synopsis

Abstract

The surface of a gold (Au) disk electrode was modified with a self‐assembled monomolecular layer of dithiobis(4‐butylamino‐m‐phenylboronic acid) (DTBA‐PBA) to prepare L‐lactate‐sensitive electrodes. The DTBA‐PBA‐modified electrodes exhibited an attenuated cyclic voltammogram (CV) for the Fe(CN)~6~^3−^ ion in the presence of L‐lactate, as a result of the formation of phenylboronate ester of L‐lactate accompanied with the addition of OH^−^ ion to the boron atom. In other words, the negatively charged DTBA‐PBA monolayer blocked the electrode surface from the access of the Fe(CN)~6~^3−/4−^ ions. Thus, the DTBA‐PBA monolayer‐modified Au electrode can be used for determining L‐lactate on the basis of the change in redox current of Fe(CN)~6~^3−/4−^ ions. The calibration graph useful for determining 1–30 mM L‐lactate was obtained.