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Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

✍ Scribed by Martin Bartošík; Miroslav Fojta; Emil Paleček


Book ID
116445380
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
550 KB
Volume
78
Category
Article
ISSN
0013-4686

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Analysis of 5-methylcytosine in DNA: I.
✍ Joseph Deutsch; Aharon Razin; John Sedat 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 419 KB

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in +x 174 DNA, in a sample of less than 10 pg and requires les