Electrical microenvironment influence on the hydrolytic activity of free and immobilisedYarrowia lipolyticalipase

✍ Scribed by M. Paquot; M. Deleu; C. Beaufils; C. Blecker

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 356 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00137814

✦ Synopsis

The role of electrical properties of interfaces upon the activity of free and immobilised Ynrrou~ia lipolytica lipase has been investigated. Sodium taurocholate and Sedipur 400, an anionic polyacrylamide, enhance the negative character of the fatty droplets of substrate and tend to improve the lipolytic activity while the cationic polyacrylamide (Sedipur 900) has opposite effects. Ca I which reduces the fatty droplets charge as Sedipur 900, is however a good activator of the enzyme. The role of electrical properties on the optimum pH of the immobilised enzyme is clearer. Immobilisation of the lipase on a positively charged support shifts its optimum pH to acidic pH by repulsion towards H' ions around the support.