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Ehrlich ascites tumor cell surface labeling and kinetics of glycocalyx release

✍ Scribed by Smith, Thomas C. ;Levinson, Charles

Publisher: Wiley (John Wiley & Sons)
Year: 1979
Tongue: English
Weight: 703 KB
Volume: 12
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400120109

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✦ Synopsis

Ehrlich ascites tumor cells spontaneously release cell surface material (glycocalyx) into isotonic saline medium. Exposure of these cells to tritium-labeled 4,4'-diisothiocyano-1,2-diphenylethane-2,2'-disulfonic acid ( H2 DIDS) at 4°C' leads to preferential labeling of the cell surface coat. We have combined studies of the kinetics of H2 DIDS-label release, the effects of enLymatic treatment, and cell electrophoretic mobility to characterize the 1-12 DIDSlabcled components of the cell surface. Approximately 7 ted radioactivity is spontaneously released from the cells The kinetics of release is consistent with the first-order loss of t w o fractions; a slow

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