A kinetic analysis of purine nucleotide synthesis and interconversion in intact Ehrlich ascites tumor cells
✍ Scribed by Floyd F. Snyder; J. Frank Henderson
- Publisher
- John Wiley and Sons
- Year
- 1973
- Tongue
- English
- Weight
- 912 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
A pseudo‐first order kinetic analysis has described the reactions of purine ribonucleotide synthesis and interconversion in Ehrlich ascites tumor cells in vitro. Rate constants were determined for 13 reactions at non‐saturating precursor concentration (50 μM); nucleotide formation from adenine‐^14^C and hypoxanthine‐^14^C was rate limiting for ATP synthesis and nucleotide formation from guanine‐^14^C and hypoxanthine‐^11^C was rate limiting for GTP synthesis. The initial rites of nucleotide synthesis from 1.0 mM adenine‐^14^C and hypoxanthine‐^14^C were equivalent to the rates of nucleotide synthesis at non‐saturating base concentration, but the rate of nucleotide synthesis at saturating base concentration after 30 minutes incubation was 20‐25% that of non‐saturating precursor concentration.