Efflux of inorganic phosphate from rat hepatocytes: Lack of effect of insulin

✍ Scribed by Mukesh Parkash; Peter J. Butterworth

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 276 KB
• Volume: 9
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290090103

✦ Synopsis

Efflux of Pi from rat hepatocytes suspended in phosphate free-medium was studied by chemical assay of released Pi and by monitoring the loss in radioactivity of cells pre-labelled with ["PI-Pi. The release follows first-order kinetics fairly closely with a rate constant of 7 x 10e3min-approximately. Insulin at a concentration of ~O -* M had no effect on the rate of Pi release and it is concluded therefore, that the insulin-stimulated accumulation of Pi described in the literature is the result of hormone action on Pi uptake by liver rather than on its release.