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Separation of 32P-labeled inorganic phosphate from rat liver homogenates

โœ Scribed by Robert F. Derr; Rodney Olsen; Leslie Zieve


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
251 KB
Volume
77
Category
Article
ISSN
0003-2697

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Interaction of homogeneous mitochondrial
โœ Pedersen, Peter L. ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 480 KB

## Abstract Mitochondrial ATPase from rat liver mitochondria contains multiple nucleotide binding sites. At low concentrations ADP binds with high affinity (1 mole/mole ATPase, K~D~ = 1โ€“2 ฮผM). At high concentrations, ADP inhibits ATP hydrolysis presumably by competing with ATP for the active site (