The apparent kinetics of uptake of various model substrates were examined for hepatocytes in suspension and primary culture up to 72 h. The ability of hepatocytes to take up taurocholate and ouabain was decreased in culture. Vmax for uptake of both substrates diminished rapidly with increasing time
Kinetics of taurocholate efflux from freshly isolated suspensions and primary cultures of rat hepatocytes
β Scribed by Veerapol Kukongviriyapan; Dr. Neill H. Stacey
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 754 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0270-9139
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To differentiate between the "intact" and "sick" cell hypoβ¬he& explaining decreased clearance of endo-and xenobbtics, we meaeured uptake of taurocholate and OIlQBOill in hepatocytes isolated from cirrhotic rat liver. Cirrhouis was induced by chronic exposure of male Sprague-Dawley rats to phenobarbi