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Decreased uptake of taurocholate and ouabain by hepatocytes isolated from cirrhotic rat liver

✍ Scribed by Jürg Reichen; Catherine Hoilien; Mysan Le; Richard H. Jones

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
454 KB
Volume
7
Category
Article
ISSN
0270-9139

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✦ Synopsis

To differentiate between the "intact" and "sick" cell hypo€he& explaining decreased clearance of endo-and xenobbtics, we meaeured uptake of taurocholate and OIlQBOill in hepatocytes isolated from cirrhotic rat liver. Cirrhouis was induced by chronic exposure of male Sprague-Dawley rats to phenobarbital and carbon tetrcschloride. Uptake of 1 "C]taurocholate and ['Hlouabain was measpred by a rapid fiitration technique. Hepatocytea from cirrhotic liver were as viable as control hepatocytee-as judged by trypan blue exclusion and lactate dehydmgenase release-but consumed 28% lees oxygen. V , of both taurocholate (3.16 2 0.96 vs. 0.40 f 0.35 nmoles.min-lo 10' cells-'; p c 0.001) and ouabain (2.16 f 0.78 vs. 0.83 f 0.26 nmoles.min-I-10' cells-'; p < 0.005) was significantly reduced. These results are compatible with the "sick" cell hypothesis.

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