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Efficient synthesis of oligoribonucleotide and its phosphorothioate analogue using H-phosphonate approach

✍ Scribed by Sudhir Agrawal; J.-Y. Tang

Publisher: Elsevier Science
Year: 1990
Tongue: French
Weight: 334 KB
Volume: 31
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(00)97293-9

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✦ Synopsis

Efticient solid phase synth8sis of oligorfbonucleotiie and its phosphorothioate analogue is described that utilizes the dimethoxytrityl (DMTr) for 5'-protection and t-butyldimethylsilyl (t-BDMS) group for P'-protection of ribonucleoside monomers and th8 H-phosphonate coupling procedure. The synthetic cycles have been optimised to use only 8-10 fold excess of monomer at each coupling step, leading to an average coupling yield of 97%.

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