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Efficient isolation of total RNA from Clostridium without DNA contamination

✍ Scribed by S. Nuyts; L. Van Mellaert; P. Lambin; J. Anné

Book ID
117359755
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
71 KB
Volume
44
Category
Article
ISSN
0167-7012

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Rapid and simple removal of contaminatin
Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase
✍ Michael V. Norgard 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 859 KB

A procedure for the removal of RNA and RNA fragments from large quantities of pBR322 plasmid DNA without the use of RNase is described. Sephacryl S-300 is employed for the separation of low-molecular-weight RNA from plasmid DNA molecules on the basis of gel filtration. The technique thus circumvents