Efficient isolation inner cell mass from blastocysts by improved microsurgical technique
β Scribed by Ding, Yan; Gan, Yi; Feng, Jing Bo; Qi, Shao Hua; Li, Rui Ming; Li, Dong Sheng
- Book ID
- 109846673
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 238 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0007-0610
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## Abstract The developmental potential of the inner cell mass (ICM) of the cultured mouse embryo was determined by testing the ability of the ICM to regenerate trophoblast in vitro. ICM's isolated by immunosurgery from either single or chimeric embryos were able to regenerate trophoblast when they
## Abstract Mouse ICM's that were isolated from blastocysts by immunosurgery and subsequently cultured for four to five days were tested for their ability to form trophoblast giant cells (TGC's) in vitro. The cores were isolated from such ICM's by a second immunosurgery and cultured for 7 to 14 day