✦ LIBER ✦

Effects of water activity on Vmaxand KMof lipase catalyzed transesterification in organic media

✍ Scribed by Roberto Bovara; Giacomo Carrea; Gianluca Ottolina; Sergio Riva

Publisher: Springer Netherlands
Year: 1993
Tongue: English
Weight: 418 KB
Volume: 15
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00131760

No coin nor oath required. For personal study only.

✦ Synopsis

The V,, and KM of various forms of lipase from Pseudomonas cepacia ( powder, adsorbed onto Celite or covalently linked to polyethylene glycol ) were determined in organic solvents preequilibrated to water activities ( a, ) from ~0.1 to 0.84. The model reaction was the transesterification between n-octanol and vinyl butyrate. It was found that Ki,., for the nucleophile increased with increasing a, for all three lipase forms. V,, increased with increasing a, for polyethylene glycol-lipase, whereas there was an optimum at intermediate a, values ( 0.11 -0.38 ) for lipase powder and Celite-immobilized lipase.

📜 SIMILAR VOLUMES

Effect of solvent on lipase-catalyzed tr

Effect of solvent on lipase-catalyzed transesterification in organic media

✍ Kaoru Nakamura; Masamichi Kinoshita; Atsuyoshi Ohno 📂 Article 📅 1994 🏛 Elsevier Science 🌐 French ⚖ 542 KB

Lipase-catalyzed transesterifications of cis-and Iran.+4-methylcyclohexanols with vinyl acetate in various organic solvents have been studied, and the cffcct of' solvent on activity and stereoselectivity of lipase has been investigated. Initial rate of the reaction increases with the increase in hyd

Lipase-catalyzed transesterification in

Lipase-catalyzed transesterification in organic media: Solvent effects on equilibrium and individual rate constants

✍ Luis F. García-Alles; Vicente Gotor 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB 👁 2 views

The kinetics of the immobilized lipase B from Candida antarctica have been studied in organic solvents. This enzyme has been shown to be slightly affected by the water content of the organic media, and it does not seem to be subject to mass transfer limitations. On the other hand, some evidence indi

Synthesis of alkyds involving regioselec

Synthesis of alkyds involving regioselective lipase-catalyzed transesterification in organic media

✍ G. Sudesh Kumar; A. Ghogare; D. Mukesh 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 687 KB

Porcine pancreatic Iipase (PPL) is established as an effective biocatalyst for the selective transesterification of triglycerides and diols in organic medium at room temperature yielding primarily 2-monoglyceride mixtures. Molecular modelling simulations of repeating units of alkyd resin incorporati

Effect of lipase conditioning on its act

Effect of lipase conditioning on its activity in organic media

✍ Fréderic Monot; Emmanuelle Paccard; Frédérique Borzeix; Marc Bardin; Jean-Paul V 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 473 KB

Significant effect of acyl groups on ena

Significant effect of acyl groups on enantioselectivity in lipase-catalyzed transesterifications

✍ Tadashi Ema; Soichi Maeno; Yusuke Takaya; Takashi Sakai; Masanori Utaka 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 243 KB

The effect of the acyl group of acylating agents on the enantioselectivity in the lipasecatalyzed transesterifications of racemie 2-[(N,N-dimethylearbamoyl)methyl]-3-eyelopenten-l-ol in diisopropyl ether was found 1o be significant. The enantioselectivity was enhanced markedly by changing the aeylat

Effect of temperature on lipase-catalyze

Effect of temperature on lipase-catalyzed esterification in organic solvent

✍ Yoshitaka Yasufuku; Shin-ichi Ueji 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 254 KB

For the esterification of 2-(4-ethylphenoxy)propionic acid catalyzed by lipase MY (Candida rugosa) in isopropyl ether containing a suitable amount of water, the enantioselectivity for the reaction has become higher asthe reaction temperature increasing. In contrast, the reverse trend of the tempera