The V,, and KM of various forms of lipase from Pseudomonas cepacia ( powder, adsorbed onto Celite or covalently linked to polyethylene glycol ) were determined in organic solvents preequilibrated to water activities ( a, ) from ~0.1 to 0.84. The model reaction was the transesterification between n-o
Effect of lipase conditioning on its activity in organic media
✍ Scribed by Fréderic Monot; Emmanuelle Paccard; Frédérique Borzeix; Marc Bardin; Jean-Paul Vandecasteele
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 473 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1432-0614
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Silica glass-entrapped lipase was prepared by the sol-gel method using tetramethoxysilane, and its esterification activity in n-hexane was examined for isoamylbutyrate formation. The hydrogel preparation containing a large amount of water exhibited enough activity. Although the activity of xerogel-e
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