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Effects of warming rate, temperature, and antifreeze proteins on the survival of mouse spermatozoa frozen at an optimal rate

โœ Scribed by Chihiro Koshimoto; Peter Mazur


Book ID
117088740
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
181 KB
Volume
45
Category
Article
ISSN
0011-2240

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## Abstract Frozen mouse eggs were examined to determine the effects of low temperatures, concentration of cryoprotective agents and cooling rates on their survival, fertilizability in vitro and subsequent development. Dimethyl sulfoxide administered at 1.5 M concentration was found to be the most