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Effects of prefreezing temperature, freezing rate and cryoprotectants on the survival of apical meristems of Vaccinium frozen in liquid nitrogen

✍ Scribed by Barbara M. Reed; H.B. Lagerstedt


Book ID
115850710
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
235 KB
Volume
24
Category
Article
ISSN
0011-2240

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## Abstract Frozen mouse eggs were examined to determine the effects of low temperatures, concentration of cryoprotective agents and cooling rates on their survival, fertilizability in vitro and subsequent development. Dimethyl sulfoxide administered at 1.5 M concentration was found to be the most