Effects of thiamine and pyridoxine on respiratory activity inSaccharomyces carlsbergensisstrain 4228

✍ Scribed by Ichiro Nakamura; Naohiko Isobe; Teijiro Kamihara; Saburo Fukui

• Publisher: Springer
• Year: 1980
• Tongue: English
• Weight: 458 KB
• Volume: 127
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00414354

✦ Synopsis

Studies were made to elucidate the relationship among the thiamine-induced growth inhibition, decrease in cellular vitamin B 6 content and respiratory deficiency in Saccharomyces carlsbergensis strain 4228 [Nakamura et al., Biochem. Biophys: Res. Commun. 59, 771-776 (1974)]. Addition of pyridoxine to the thiamine-added culture at the beginning or in the course of cultivation brought about appearance of cytochrome spectra and the increase in the activity of heme-containing enzymes and in respiratory activity (Qo2)-The effects of pyridoxine occurred prior to the restoration of growth. Pyridoxine was effective even in the presence of high levels of glucose in the growth medium (not less than 3%). On the basis of these results, the mechanism of the effects of thiamine and pyridoxine was discussed.