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Hydrocarbon assimilation byCandida lipolytica: Formation of a biosurfactant; Effects on respiratory activity and growth

✍ Scribed by A. Pareilleux


Publisher
Springer
Year
1979
Tongue
English
Weight
440 KB
Volume
8
Category
Article
ISSN
1432-0614

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✦ Synopsis


Candida lipolytica is shown to produce an extracellular polymer with emulsifying properties when grown on n-tetradecane or a mixture of linear hydrocarbons. A device for biosurfactant isolation is presented. The polymers recovered from the fermentation broth were found to be complex molecules with a protein, a lipid and a carbohydrate moiety. Their surface active properties suggest a possible role in hydrocarbon uptake by cells. Effectively, the addition of crude polymer resulted in an enhancement of respiration rate which was dependent on n-alkane concentration with glucose grown cells. Likewise in batch culture, maximum growth rate, cell productivity and yield were increased by the presence of the biosurfactant.