Effects of Supercoiling in Electrophoretic Trapping of Circular DNA in Polyacrylamide Gels
✍ Scribed by Åkerman, Björn
- Book ID
- 119411856
- Publisher
- Biophysical Society
- Year
- 1998
- Tongue
- English
- Weight
- 274 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0006-3495
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There are excellent methods available for separating superhelical DNA (covalently closed rings) from a mixture of open circular DNA (rings with one or more single-strand interruptions) and linear DNA (1,2). The separation of open circular DNA from linear DNA presents a more difficult problem. While
## Abstract The electrophoretic migration of rigid rodlike DNA structures with well defined topologies has been investigated in polyacrylamide (PA) hydrogels prepared by copolymerization of acrylamide and __N, N__′‐methylenebisacrylamide. Previous studies have reported structural and dynamic charac