Electrophoretic capture of circular DNA in gels
✍ Scribed by Björn Åkerman; Kenneth D. Cole
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 170 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
There are excellent methods available for separating superhelical DNA (covalently closed rings) from a mixture of open circular DNA (rings with one or more single-strand interruptions) and linear DNA (1,2). The separation of open circular DNA from linear DNA presents a more difficult problem. While
The field free diffusion constant and the electric field dependence of the electrophoretic mobility and molecular orientation of DNA samples from 5 to 164 kilobase pairs in agarose gels from 0.5 to 2% have been measured by fluorescence recovery after photobleaching and birefringence. In conditions w