Electrophoretic mobility of DNA in gels. III. Experimental study on band inversion
β Scribed by Christoph Heller; Jean Louis Viovy
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1995
- Tongue
- English
- Weight
- 545 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0006-3525
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The electrophoretic mobility ( p ) of DNA fragments from X phage and @X 174, split by restriction enzyme to molecular lengths from 3 X lo2 to 2.36 X lo4 base pairs, has been investigated in 0.6-41 agar-gels at various field strengths, ionic strengths, and temperatures. As already observed, p is see
## Abstract The electrophoretic migration of rigid rodlike DNA structures with well defined topologies has been investigated in polyacrylamide (PA) hydrogels prepared by copolymerization of acrylamide and __N, N__β²βmethylenebisacrylamide. Previous studies have reported structural and dynamic charac
We have investigated several experimental factors which affect the accurate determination of electrophoretic mobilities of circular and linear DNAs in agarose gels. We demonstrate that: (1) The mobility of individual DNA species is affected by the total mass in the sample loaded. The increased mobil