✦ LIBER ✦

Electrophoresis of DNA in agarose gels: II. Effects of loading mass and electroendosmosis on electrophoretic mobilities

✍ Scribed by Paul H. Johnson; Michael J. Miller; Lawrence I. Grossman

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 514 KB
Volume: 102
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90332-2

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✦ Synopsis

We have investigated several experimental factors which affect the accurate determination of electrophoretic mobilities of circular and linear DNAs in agarose gels. We demonstrate that: (1) The mobility of individual DNA species is affected by the total mass in the sample loaded. The increased mobility and band distortion observed become apparent when the DNA mass exceeds approximately 0.2 pg per 0.15 cm* of surface area in the loading well.

(2) The migration velocity of a given DNA species depends on the coefficient of electroendosmosis (-m,) of the agarose preparations used. In the range 0.081 s -m, I 0.441, the DNA migration velocity is proportional to (-m ,)-".S.

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