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Influence of λ-DNA concentration on mobilities and dispersion coefficients during agarose gel electrophoresis

✍ Scribed by Nadine Pernodet; Bernard Tinland

Publisher: Wiley (John Wiley & Sons)
Year: 1997
Tongue: English
Weight: 118 KB
Volume: 42
Category: Article
ISSN: 0006-3525
DOI: 10.1002/(sici)1097-0282(19971005)42:4<471::aid-bip9>3.0.co;2-n

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✦ Synopsis

experimentally that during gel electrophoresis the mobility m and the dispersion coefficient D x [reflecting band broadening; G. W.

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DNA electrophoresis in agarose gels: Effects of field and gel concentration on the exponential dependence of reciprocal mobility on DNA length

✍ Randolph L. Rill; Afshin Beheshti; David H. Van Winkle 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 190 KB 👁 2 views

Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa