𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of pyruvate on the growth of normal and transformed hamster embryo fibroblasts

✍ Scribed by Donald A. Sens; Bruce Hochstadt; Harold Amos

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
706 KB
Volume
110
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The growth of NIL and NILpy hamster embryo fibroblasts was determined in the presence and absence of pyruvate as a component of the growth medium. It was demonstrated that NIL cells respond to the presence of pyruvate by decreasing cell doubling time, glucose utilization, glutamine utilization, and increasing lactate production with the effects being more pronounced at low inoculum densities. Polyoma‐virus transformed NIL cells (NILpy) demonstrate none of the above effects upon pyruvate addition regardless of initial cell inoculum density.

📜 SIMILAR VOLUMES

Expression of growth-regulated genes in
Expression of growth-regulated genes in normal and SV40 transformed hamster fibroblasts
✍ Ann Marie Krueger Rossi; Ricky R. Hirschhorn 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 862 KB

Transformation by the oncogenic virus SV40 has been shown to alter the expression of cellular genes at the level of RNA abundance. Many of these genes have yet to be identified. We have determined, by Northern blot analysis, the abundance levels of several growth-regulated genes in SV40-transformed

Antiproliferative effects of heparin on
Antiproliferative effects of heparin on normal and transformed NIH/3T3 fibroblasts.
✍ Simone Cavari; Marco Ruggiero; Simonetta Vannucchi 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 306 KB

## Abstract We studied the effect of heparin on proliferation and signalling in normal NIH/3T3 fibroblasts, and in cells transformed by different oncogenes. Heparin inhibited the proliferation of normal as well as of v‐sis and v‐erbB transformed fibroblasts in the presence of serum, but failed to i

The growth capacity of normal and Rous-v
The growth capacity of normal and Rous-virus-transformed chicken fibroblasts in vitro
✍ Jan Pontén 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 French ⚖ 655 KB

## Abstract The proliferation dynamics of chicken embryo cells after transformation by Rous sarcoma virus, Schmidt‐Ruppin strain (RSV‐SR) has been investigated in vitro. The optimal schedule for serial cultivation for normal as well as transformed cells was to subcultivate the cells every third day

Synergistic effects of epidermal growth
Synergistic effects of epidermal growth factor and thrombin on the growth stimulation of diploid chinese hamster fibroblasts
✍ Paul V. Cherington; Arthur B. Pardee 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 640 KB

## Abstract The serum supplement used in the culture of a variety of mammalian cells can be replaced by known growth factors. Diploid Chinese hamster fibroblasts (CHEF/18) will grow for several days in a medium (4F) supplemented with four growth factors: epidermal growth factor (EGF), insulin, fibr

Transport and phosphorylation of hexoses
Transport and phosphorylation of hexoses in normal and rous sarcoma virus-transformed chick embryo fibroblasts
✍ Malathy Singh; Vishwa Nath Singh; J. T. August; Dr. B. L. Horecker 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 580 KB

## Abstract Effects of transformation by Rous sarcoma virus on sugar uptake and activity and the subcellular distribution of hexokinase isozymes in chick embryo fibroblasts were examined. Transformation caused a several‐fold increase in the maximum velocity for uptake of 2‐deoxyglucose without a si

Calcium effects on epidermal growth fact
Calcium effects on epidermal growth factor receptor-mediated endocytosis in normal and SV40-transformed human fibroblasts
✍ Joseph T. Tupper; Peter V. Bodine 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 786 KB

## Abstract Lowering of extracellular Ca^2+^ levels will reversibly arrest the growth of human fibroblasts (WI38). Simian virus~40~(SV~40~)‐transformed WI38 cells do not exhibit this Ca^2+^‐dependent arrest. One possibility for this difference in Ca^2+^ requirement is that extracellular or surface