✦ LIBER ✦

Synergistic effects of epidermal growth factor and thrombin on the growth stimulation of diploid chinese hamster fibroblasts

✍ Scribed by Paul V. Cherington; Arthur B. Pardee

Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 640 KB
Volume: 105
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041050105

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The serum supplement used in the culture of a variety of mammalian cells can be replaced by known growth factors. Diploid Chinese hamster fibroblasts (CHEF/18) will grow for several days in a medium (4F) supplemented with four growth factors: epidermal growth factor (EGF), insulin, fibroblast growth factor (FGF), and transferrin. The growth rate is only about 50% as fast as when fetal calf serum is added. This difference is eliminated by thrombin (10–100 ng/ml; 0.3–3 nM). The CHEF/18 cell line is unique in that no other cell line responds to thrombin in this concentration range.

Thrombin acts synergistically with other growth factors to stimulate CHEF/18 cell growth. By itself, thrombin is only mitogenic at elevated concentrations. Thrombin can largely compensate for the absence of EGF and partly for the absence of insulin in serum‐free media. Chemically and “spontaneously” transformed cell lines related to CHEF/18 have lost requirements for both EGF and thrombin, and have retained requirements for insulin and transferrin expressed by CHEF/18. No CHEF cells in this work required FGF. These results suggest that the mechanisms by which EGF and thrombin stimulate cells to grow are related.

📜 SIMILAR VOLUMES

Effect of prostatic growth factor, basic

Effect of prostatic growth factor, basic fibroblast growth factor, epidermal growth factor, and steroids on the proliferation of human fetal prostatic fibroblasts

✍ Luo, Daochun; Lin, Yuqin; Liu, Xianxi; Qin, Zhijie; Zhao, Chunhua; Zhang, Yuyi; 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 553 KB

To study the relationship between androgen metabolism and the pathogenesis of benign prostatic hypertrophy, we purified a growth factor from benign hyperplastic tissue of human prostates and assayed the proliferative responses of human fetal prostatic fibroblasts to the purified growth factor (hPGF)

The effect of hypoxia on acquired drug r

The effect of hypoxia on acquired drug resistance and response to epidermal growth factor in chinese hamster lung fibroblasts and human breast-cancer cells in vitro

✍ R. Kalra; A-M. Jones; J. Kirk; G. E. Adams; I. J. Stratford 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 French ⚖ 646 KB

## Abstract Prolonged hypoxia induced transient drug resistance in Chinese hamster lung fibroblasts. Previously hypoxic cells were resistant to adriamycin and resistant to etoposide. Complete recovery of etoposide sensitivity was observed following re‐aeration for 24 hr. A change in P‐glycoprotein

Effects of pyruvate on the growth of nor

Effects of pyruvate on the growth of normal and transformed hamster embryo fibroblasts

✍ Donald A. Sens; Bruce Hochstadt; Harold Amos 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 706 KB

## Abstract The growth of NIL and NILpy hamster embryo fibroblasts was determined in the presence and absence of pyruvate as a component of the growth medium. It was demonstrated that NIL cells respond to the presence of pyruvate by decreasing cell doubling time, glucose utilization, glutamine util

EFFECTS OF ADENOSINE AND EPIDERMAL GROWT

EFFECTS OF ADENOSINE AND EPIDERMAL GROWTH FACTOR ON THE GROWTH OF MOUSE MAMMARY EPITHELIAL CELLS

✍ I.S YUH; B.B JANG; B.J HONG 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 174 KB

The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine

Differential effects of basic fibroblast

Differential effects of basic fibroblast growth factor, epidermal growth factor, transforming growth factor-α, and insulin-like growth factor-I on a hypothalamic gonadotropin-releasing hormone neuronal cell line

✍ Alejandra Ochoa; Clelia Domenzáin; Carmen Clapp; Gonzalo Martínez de la Escalera 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 418 KB 👁 2 views

Recent studies in several neuronal lineages suggest that extrinsic factors such as polypeptide growth factors regulate various stages of neuronal development, from initial commitment of multipotent progenitors to induction of specific gene expression that is characteristic of terminal neuronal diffe

Effects of retinoic acid on the binding

Effects of retinoic acid on the binding and mitogenic activity of epidermal growth factor

✍ Anton M. Jetten 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 585 KB

## Abstract In this study the effects of retinoic acid on the binding and mitogenic activity of epidermal growth factor (EGF) in mouse fibroblast Balb/c 3T6 cells are further examined. Retinoic acid treatment of 3T6 cells results in a sixfold enhancement of ^125^I‐labeled mouse EGF binding when ass