With signalβtoβnoise ratio enhancements on the order of 10,000βfold, hyperpolarized MRSI of metabolically active substrates allows the study of both the injected substrate and downstream metabolic products __in vivo__. Although hyperpolarized [1β^13^C]pyruvate, in particular, has been used to demons
Effects of pyruvate dose on in vivo metabolism and quantification of hyperpolarized 13C spectra
β Scribed by M. A. Janich; M. I. Menzel; F. Wiesinger; E. Weidl; O. Khegai; J. H. Ardenkjaer-Larsen; S. J. Glaser; A. Haase; R. F. Schulte; M. Schwaiger
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 421 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0952-3480
- DOI
- 10.1002/nbm.1726
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## Abstract Dynamic nuclear polarization can be used to increase the sensitivity of solution state ^13^C magnetic resonance spectroscopy by four orders of magnitude. We show here that [1β^13^C]glutamate can be polarized to 28%, representing a 35,000βfold increase in its sensitivity to detection at
We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1β^13^C pyruvate to compare the rate of lactate labeling differing only in the temporal order
A single-voxel Carr-Purcell-Meibloom-Gill sequence was developed to measure localized T(2) relaxation times of (13)C-labeled metabolites in vivo for the first time. Following hyperpolarized [1-(13)C]pyruvate injections, pyruvate and its metabolic products, alanine and lactate, were observed in the l