Metabolism of hyperpolarized [1-13C]pyruvate in the isolated perfused rat lung – an ischemia study

✍ Scribed by B. Pullinger; H. Profka; J. H. Ardenkjaer-Larsen; N. N. Kuzma; S. Kadlecek; R. R. Rizi

• Publisher: John Wiley and Sons
• Year: 2012
• Tongue: English
• Weight: 204 KB
• Volume: 25
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/nbm.2777

✦ Synopsis

We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1‐^13^C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several‐fold increase in lactate labeling was observed immediately after a 25‐min ischemia event as was its reversal back to the baseline after 30–40 min of resumed perfusion (n = 5, p < 0.025 for both comparisons). These results were corroborated by ^31^P spectroscopy and correspond well to measured changes in lactate pool size determined by ^1^H spectroscopy of freeze‐clamped specimens. Copyright © 2012 John Wiley & Sons, Ltd.