Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell
Effects of low frequency pulsed electrical current on keratinocytes in vitro
โ Scribed by M. Hinsenkamp; A. Jercinovic; Ch. de Graef; F. Wilaert; M. Heenen
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 253 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0197-8462
No coin nor oath required. For personal study only.
โฆ Synopsis
The effects of low frequency pulsed electrical current on epidermal repair in vitro were examined. Chargebalanced current stimuli proposed for chronic wound treatment were tested on skin keratinocytes cultured at an air-liquid interface on dead human dermis. Results imply that the balance between proliferation and differentiation in electrically treated samples is significantly modified in favor of differentiation. More advanced differentiation, shown through epidermal histology, was obtained in cultures exposed to electrical current, whereas the culture growth, the result of keratinocyte migration and proliferation, was greater in control samples.
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