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Effects of ion species in aqueous phase on protein extraction into reversed micellar solution

✍ Scribed by Takumi Kinugasa; Aki Kondo; Emiko Mouri; Sakiko Ichikawa; Satomi Nakagawa; Yasuhiro Nishii; Kunio Watanabe; Hiroshi Takeuchi


Book ID
114397021
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
228 KB
Volume
31
Category
Article
ISSN
1383-5866

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A water-soluble polymer such as polyethylene glycol (PEG), Dextran T-500 (Dx), or diethylaminoethyl-Dextran (DEAE-Dx) induced aqueous micellar solutions of octyl-␀-D-thioglucoside (OTG) to phase separation at 0°C. One of the two phases thus formed is a surfactantdepleted aqueous solution (aqueous ph