✦ LIBER ✦

Effects of ion mode and matrix additives in the identification of bacteria by intact cell mass spectrometry

✍ Scribed by David J. Evason; Martin A. Claydon; Derek B. Gordon

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 62 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(20000430)14:8<669::aid-rcm932>3.0.co;2-7

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✦ Synopsis

Protocols for the identification of bacterial cells by intact cell matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (ICM-TOFMS) are presented. A mass range of 500 to 10 000 m/z is used. The use of formic acid and the crown ether 1, 4, 7, 10, 13, 16-hexaoxacyclooctadecane (18-crown-6) is described. Crown ether is useful for removing metal ion adducts, which degrade spectral purity, and formic acid promotes positive ions, improves spectral signal, and, hence, increases identification certainty.

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