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EFFECTS OF INTERLEUKIN-6 ON PROLIFERATION AND PROTEOGLYCAN METABOLISM IN ARTICULAR CHONDROCYTE CULTURES

✍ Scribed by Akitoshi Jikko; Takashi Wakisaka; Masahiro Iwamoto; Hiroko Hiranuma; Yukio Kato; Takashi Maeda; Masami Fujishita; Hajime Fuchihata

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
204 KB
Volume
22
Category
Article
ISSN
1065-6995

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✦ Synopsis

Interleukin-6 (IL-6) levels are markedly increased in the synovial fluid of patients with rheumatoid arthritis or osteoarthritis. However, the effects of IL-6 on proliferation and proteoglycan metabolism in articular cartilage are not known. We demonstrated here the effects of human recombinant (hr) IL-6 on proliferation and proteoglycan metabolism in rabbit articular chondrocyte cultures. In vitro, these cells proliferated and produced abundant extracellular matrices. We found that 1-10 ng/ml of hrIL-6 inhibited proliferation to approximately 65% of control levels and suppressed colony formation induced by bFGF in soft agarose. The same concentration of hrIL-6 depressed proteoglycan synthesis to approximately 60% of control levels. Moreover, hrIL-6 significantly enhanced proteoglycan degradation induced by hrIL-1beta, although hrIL-6 alone did not affect proteoglycan degradation. These findings suggest that IL-6 is a negative regulator for chondrocyte proliferation and articular cartilage metabolism.

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