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Effects of oxygen-free radicals on proliferation kinetics of cultured rabbit articular chondrocytes

✍ Scribed by FrançOise Vincent; Hervé Brun; Elisabeth Clain; Xavier Ronot; Monique Adolphe

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
495 KB
Volume
141
Category
Article
ISSN
0021-9541

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✦ Synopsis

It can be postulated that among the factors implicated in cartilaginous lesions, oxygen-derived free radicals seem to have a prominent part. To investigate this hypothesis, rabbit articular chondrocyte cultures have been exposed to oxygenderived reactive species generated by the hypoxanthine-xanthine oxydase systern. We observed a dose-dependent decrease of cellular growth. In order to explain this result, cell cycle progression and binucleate cell fractions have been studied. A greater number of binucleate cells and an increase in cell volume were observed. Flow cytornetry analysis revealed a perturbation in cell cycle progression leading to a significant increase in the proportion of cells in C2 phase and an important augmentation in cell protein content confirmed by biochemical assays.

This model shows which type of alteration can be induced by oxygen-derived free radicals in vitro. In addition, we deem this model to be useful for studying degenerative processes and for screening drugs that can scavenge oxygen-free radicals.

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