The validation of a simple and rapid DNA solubilization procedure is described. Quantitative extraction of intact, polymerized DNA was achieved by cell lysis or tissue homogenization in an ammonium hydroxide-Triton X-100 solution. The solubilization procedure inactivates endogenous DNAase and increa
โฆ LIBER โฆ
Effects of humic substances on fluorometric DNA quantification and DNA hybridization
โ Scribed by Dave S. Bachoon; Ernesto Otero; Robert E. Hodson
- Book ID
- 117359823
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 143 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0167-7012
No coin nor oath required. For personal study only.
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๐ 2 views
## Abstract DNA from bacteriophage T7 was irradiated at long ultraviolet wavelengths in the presence of silver ions. Such treatment leads to selective production of thymine: thymine dimers in DNA. The DNA was melted and the renaturation rate was determined as a function of thymine dimer content and