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Comparison of fluorometric and spectrophotometric DNA quantification for real-time quantitative PCR of degraded DNA

✍ Scribed by Luke A. Shokere; Marcia J. Holden; G. Ronald Jenkins


Book ID
116485761
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
589 KB
Volume
20
Category
Article
ISSN
0956-7135

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## Abstract A sensitive and reproducible real‐time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an β€œin‐house” qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B pati