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Effects of feeding and induction strategy on the production of BmR1 antigen in recombinant E. coli

โœ Scribed by A. Norsyahida; N. Rahmah; R.M.Y. Ahmad


Book ID
111192434
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
302 KB
Volume
49
Category
Article
ISSN
0266-8254

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The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponen- tial fed-batch mode to the OD 600 of ca. 100 (48 gDCW/L) prior to induction. Six differ