Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli
✍ Scribed by Heng Ho Wong; Yeon Chul Kim; Sang Yup Lee; Ho Nam Chang
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 339 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponen- tial fed-batch mode to the OD 600 of ca. 100 (48 gDCW/L) prior to induction. Six different post-induction nutrient feeding strategies (pH-stat, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein production was examined. It was found that post-induction cell growth was independent of nutrient feeding rate. However, bioadhesive protein production was significantly affected by post-induction feeding strategies. Linearly changing post-induction feeding rate with a suitable slope allowed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies.